Treatment with Shp2 or Mek inhibitor upregulated CD133 expression in mammary epithelial cells MCF10A (Figure 4F, G), glioblastoma cells GSC3028 and GSC23, insulinoma cells MIN6, colon adenocarcinoma cells MC38, and cervical cancer cells Hela as well as kidney fibroblasts COS7 (Figure 4H). This evidence concerns the gene PTPN11 and pancreatic insulinoma.