CCR4 and esophageal squamous cell carcinoma: To further evaluate whether CCR4 participates in CCL22‐mediated Gli1 activity, rhCCL22 (50 ng/mL) was added to incubate with CCR4‐depleted KYSE410/KYSE510 cells, we found that CCR4 siRNAs effectively inhibited CCL22‐induced expression of nuclear Gli1 and its activity in indicated ESCC cells (Figure S3A,B).