We investigated whether Gli1 activation is required for the CCL22 signaling‐mediated invasiveness of ESCC cells, found that depletion of Gli1 by short‐hairpin (sh)RNA blocked the activity of Gli reporter induced by rhCCL22 and pri‐TAMs (Figure 2C,D), and inhibited the CCL22‐ or TAMs‐induced anchorage‐independent colony formation and invasion of ESCC cells (Figure 2E,F and Figure S4). This evidence concerns the gene GLI1 and esophageal squamous cell carcinoma.