Re-constructed heat map of these 12 genes (Fig. 4A).By constructing a K-M curve for the selected apoptosis-related genes, we found that the high expression of ABCB9, SPTBN2, GULP1, IGF1, P4HB, NES and DPYSL2 and the low expression of ANXA6, OAS1, RAD9a, DNASE2B and FANCF would be beneficial to the prognosis of bladder cancer patients (Fig. 4B). The gene discussed is NES; the disease is urinary bladder cancer.