CCK8 proliferation assay showed that compared with the si-NC group, the bladder cancer cells in the si-P4HB group showed significantly reduced proliferation ability (Fig. 10D); flow cytometry analysis showed increased apoptosis in bladder cancer cells in the si-P4HB group (Fig. 10E); in addition, Transwell migration and invasion assays showed that both the migration and invasion ability of the cells was reduced (Fig. 10F). This evidence concerns the gene P4HB and urinary bladder cancer.