MFN2 and kidney cancer: To further clarify the role of MFN2 in kidney cancer cells, we subjected 786O and Caki-1 cells with low MFN2 expression to overexpression experiments.After verifying the successful transfection (Fig. 11A-B), stable transfected cell lines were established, RNA and total protein were extracted, and the overexpression efficiency of MFN2 was verified by RTPCR and WB assays both suggesting successful overexpression (Fig. 11C-F).The CCK8 assay was first performed showing that overexpression of MFN2 significantly inhibited the proliferative capacity of 786O and Caki-1 cells (Fig. 12A-B).