To test whether this effect could be predicted based on TP53 mutation status and primary ciliogenesis, we used in vivo flank osteosarcoma models generated from Osx p53Rb KO mice (D12M), 45Ca mice (CaOS18), and human MG63 and B143 (ciliated) and human U2OS and SJSA (unciliated) xenograft models. The gene discussed is TP53; the disease is osteosarcoma.