We also identified potentially novel E3 ligases that were highly expressed at bulk level in tumor and specifically expressed in malignant cells at single-cell levels, such as PRAME Nuclear Receptor Transcriptional Regulator (PRAME) in lung cancer and Rho Related BTB Domain Containing 3 (RHOBTB3) in Uveal Melanoma (Supplementary Fig. 5a). This evidence concerns the gene PRAME and lung cancer.