Regarding our previous findings on Akt in mice lacking Gαi2 or Gαi3 (Dizayee et al. 2011), one should bear in mind that carbachol is rather considered a non-pathologic stimulus, and stimulation of muscarinic receptors might be beneficial under pathological conditions, e.g., heart failure (Communal et al. 1999; Olshansky et al. 2008; Lorenz et al. 2009). This evidence concerns the gene AKT1 and heart failure.