To establish the pathological relevance of SPTAN1, NUMB, MARK, WW45, and Hippo/MST1/2/YAP signals in patients with hepatocellular carcinoma (HCC), we examined 60 pairs of liver-derived tumorous and adjacent nontumorous tissues (Supplemental Figure 16A). This evidence concerns the gene SAV1 and hepatocellular carcinoma.