In the case of TRAIL‐resistant cells HT‐29, rNDV‐TRAIL infection strongly activated caspases‐8, ‐9, and ‐3 as well as the release of cytochrome c from mitochondria, surpassing the effects of rNDV infection alone I. The enhanced expression of DRs, along with theirincreased interaction with TRAIL protein, activated the extrinsic apoptotic pathway. Here, TNFSF10 is linked to infection.