52 In a previous study, we have shown in a large CRC patients’ cohort that P. micra was significantly associated with methylation of the WIF1 promoter, a very well-known TSG.24 We now report for the first time that P. micra increases global DNA methylation of target host cells using an in vitro co-culture model of human primary colonic cells. The gene discussed is WIF1; the disease is colorectal carcinoma.