Tregs suppress the T effector (T-eff) cells through inhibitory cytokines such as transforming growth factor (TGF)-β, interleukin (IL)-10, direct cytotoxicity through perforins/granzyme, promotion of T cell exhaustion and thus prevent the tumor-specific immune response which leads to resistance to ICI[24-26]. This evidence concerns the gene TGFB1 and neoplasm.