To consolidate our findings, we established an obesity-associated VCAM1 elevation model via feeding mice with an HFD, and changes in plasma TC, plasma TG, plasma HDL-C, plasma LDL-C, and mouse body weight confirmed the success of the animal model (Supplementary Figures S4A–E). This evidence concerns the gene VCAM1 and obesity due to melanocortin 4 receptor deficiency.