RHO and cyst: As shown in Figures 4A and 4B, after 12 days of growth Haspin inhibition through either 5-Itu or CHR-6494 caused a significant increase in cysts with more than one lumen (and, accordingly, a concomitant reduction of cyst circularity, particularly evident in cysts with multiple lumen, Figure S4B), implying a role for the Haspin-ARHGAP11A-RHO-ROCK axis in tissue morphogenesis.