There were eight objectives for this study: (1) to establish a colony of gluten-free Balb/c mice; (2) to test the intrinsic sensitization capacity of ASDG when repeatedly applied over undamaged skin by measuring IgE antibody responses; (3) to study clinical symptoms of anaphylaxis upon systemic ASDG challenge; (5) to quantify hypothermic shock responses (HSR); (6) to quantify mucosal mast cell degranulation responses (MMCR); (7) to determine inter-relationships among IgE, HSR, and MMCR; and (8) to characterize the systemic T-helper (Th)-1, and Th2 immune responses in this model. The gene discussed is IGHE; the disease is anaphylaxis.