As we previously observed in the ASNS gene methylation status evaluated with the NGS analysis [17], both basal (R2 = 0.26, p = 1.6 × 10−6; Figure 2c) and asparaginase-induced (R2 = 0.22, p = 1.0 × 10−5; Figure 2d) ASNS gene expression levels in 79 BCP-ALL cell lines were significantly correlated with mean percent methylation evaluated with the HPLC method. This evidence concerns the gene ASPG and acute lymphoblastic leukemia.