ASPG and acute lymphoblastic leukemia: By using next-generation sequence (NGS) analysis of bisulphite PCR products of genomic DNA, we have recently shown that an allele-specific methylation of the ASNS gene due to aberrant genome imprinting is highly involved in asparaginase sensitivity and is associated with karyotypes in B-precursor ALL (BCP-ALL) [17] and T-ALL [18].