To evaluate if HiFi-RfxCas13d could also prevent the formation of inclusions consisting of ALS-linked TDP-43 mutants, we transfected HEK293T cells with HiFi-RfxCas13d and crRNA-6 alongside a reporter expressing TDP-43A315T linked to YFP, which enabled us to monitor the formation of pathological aggregates. This evidence concerns the gene TARDBP and amyotrophic lateral sclerosis.