Next, we demonstrated that the natural dianthrone glycoside called sennoside A (SenA), a popular herbal laxative for constipation with molecular weight 862.74 g/mol (Figure 3B) is a small molecule inhibitor of SSH1 and that exposure to 0 - 100 μM SenA dose-dependently suppressed the viability of Huh7 (IC50 = 55.23 μM), HepG2 (IC50 = 11.27 μM), Mahlavu (IC50 = 85.06 μM), or Hep3B (IC50 = 37.86 μM) HCC cell lines (Figure 3C). This evidence concerns the gene SSH1 and hepatocellular carcinoma.