SSH1 and hepatocellular carcinoma: To better understand the oncogenic role of SSH1 in HCC, we performed a loss-of-function assay using the CRISPR/Cas9 technique and found that compared to the wild-type Huh7 (Huh7-WT) cells, cells with knocked-out SSH1 gene (Huh7-SSH1-/- (1) and (2)) exhibited significantly reduced viability (Figure 3A, left) and proliferation over time, as expressed by a ~1.3-fold reduction in the proliferation of Huh7-SSH1-/- cells (p < 0.05) (Figure 3A, right).