As the key enzymes in O-GlcNAcylation, O-GlcNAc transferase (OGT), and O-GlcNAcase (OGA), Wu et al. showed that WMW increased colonic O-GlcNAcylation levels to inhibit necroptosis and alleviate TNBS-induced colitis, as demonstrated by the elevated OGT activity and decreased OGA activity in TNBS-induced colitis in mice. Here, OGT is linked to colitis.