Again, leptin and nesfatin-1 concentrations were significantly higher in the PT group compared to the control group [mean Leptin ± SD (pg/mL): PT group = 2.88 ± 0.74, control group = 2.34 ± 0.48, Mean Nesfatin-1 ± SD (ng/dL): PT group = 0.84 ± 0.61, control group = 0.44 ± 0.28, p < 0.05)], suggesting that these findings are independent of overweight or obesity. This evidence concerns the gene LEP and obesity due to melanocortin 4 receptor deficiency.