NOS2 and serum lipopolysaccharide activity: The HFD caused intestinal permeabilization, endotoxemia, and reduced expression of tight junction proteins (occludin, ZO-1, and claudin-1) in the ileum, as well as increasing NADPH oxidase (NOX1 and NOX4), NOS2, oxidative stress, and the activation of redox-sensitive signals (NF-κB and ERK1/2).