Taken together, these results suggest that FB3-14 intervention increased GPR41 levels associated with higher levels of butyric acid content, increased the PYY and GLP-1 levels, and upregulated the mRNA relative expression of Adipor1 and Adiporq, thereby alleviating HFD-induced obesity. Here, FFAR3 is linked to obesity due to melanocortin 4 receptor deficiency.