Expression of NF-κB, TLR4, Myd88, and downstream molecules such as TNF-α, IL-6, and IL-1β was effectively reduced by SGP-H, which improved the secretion of anti-inflammatory cytokines including IL-20 and IL-10 in the colon tissue and serum of DSS-induced colitis mice. This evidence concerns the gene TLR4 and colitis.