ZNF154 was unmethylated in all non-tumor-derived cell lines (range 0–0.05) and hypermethylated in all tumor-derived cell lines (range 0.47–0.99), with the exception of Nt2d1 (a teratocarcinoma cell line), which had methylation beta values < 0.1 for all three markers. Here, ZNF154 is linked to teratocarcinoma.