The goal of this study is to conduct a comprehensive evaluation of the DNA methylation levels of the recently described set of promising DMMs, HOXA1, CLEC11A, AK055957, and TSPYL5, in liver tissues from non-cirrhotic HCC, and to compare them to cirrhotic HCC, cirrhotic livers, non-cirrhotic livers, and benign lesions (adenoma and focal nodular hyperplasia). This evidence concerns the gene TSPYL5 and hepatocellular carcinoma.