Significantly fewer Nanog-positive (1.9% vs. 34.0%, p = 0.017), SOX2-positive (0.4% vs. 6.1%, p = 0.031), and SSEA-3-positive (50.9% vs. 89.7%, p = 0.047) HSCs were detected in AML M4/M5 samples compared to NBM. The gene discussed is NANOG; the disease is acute myeloid leukemia.