TLR2 and allergic disease: To choose a suitable medium for the phenotype of interest, one must consider if the study of interest is focused on atopic asthma, asthma, allergy, perennial allergic rhinitis, seasonal allergic rhinitis, or viral reactions, where testing the c-KIT, IgE, and TLR2 expressions is crucial, culturing hCBMCs in medium B is preferred and recommended compared to medium A. On the contrary, if the focus of the study is neuropeptides, drug response, or chronic urticaria where MRGX2 is significantly increased, medium A should be considered the appropriate medium compared to medium B.