Overall, in the K. pneumoniae strains isolated from patients with hospital-acquired infections, our real-time PCR assays showed an elevated expression of adhesion genes (mrkD and ycfM) and iron-acquisition systems (entB, irp1, irp2, and fyuA) associated with the presence of antibiotic-resistance genes to beta-lactam antibiotics (blaSHV, blaCITM, blaTEM, and blaCTXM-1), sulfonamides (sul1), and tetracycline (tetA) (Table 4). This evidence concerns the gene ACO1 and nosocomial infection.