JTB and breast cancer: In this work, we aim to investigate the differentially expressed proteins (DEPs) and tumorigenic pathways associated with JTBhigh using 2D-PAGE coupled with nLC-MS/MS proteomics of MCF7 BC cell line in order to complete and complement our previously published results based on SDS-PAGE [15] and in-solution proteomics of MCF7 cells transfected for JTB upregulation [16].