JTB and breast cancer: Moreover, our present and previous results sustain the necessity of complementary use of different proteomics-based methods (SDS-PAGE, 2D-PAGE, and in-solution digestion) followed by MS analysis to avoid their inherent limitations, with each method leading to the delineation of specific clusters of DEPs that may be merged together for a better understanding of molecular pathways and neoplastic mechanisms related to JTB gene or gene product role in BC initiation and progression.