To achieve higher diagnostic precision, Siref et al. performed a dual 5-hmC/Melan-A immunohistochemical staining in 41 metastatic melanomas and 20 nodal nevi and found a total or partial loss of nuclear expression of 5-hmC in 97.56% of melanomas, while 100% of nodal nevi showed a strong expression [25]. Here, MLANA is linked to melanoma.