As a result, compared to adjacent lung tissues, we screened nine upregulated and hypomethylated hub genes (CCL20, MUC5B, ALDH3B2, TFF1, FA2H, TK1, BIRC5, ANLN and DEPDC1B) in lung adenocarcinoma which were significantly elevated, and the high expression of these genes was significantly associated with poor overall survival in patients with LUAD (Figure 2b,c). This evidence concerns the gene ANLN and lung adenocarcinoma.