A-alum-1 decreased the slightly induced MITF mRNA and protein expression levels after 24 h of α-MSH treatment (Supplementary Figure S1), suggesting that A-alum-1 decreases levels of tyrosinase mRNA and protein in the α-MSH-induced B16F1 melanoma cells via suppressing intracellular MITF mRNA and protein levels. The gene discussed is TYR; the disease is melanoma.