Furthermore, to assess the effect of antioxidants on the autophagy defect, we used the Tandem Sensor RFP- GFP-LC3B for monitoring autophagosomes and autolysosomes’ formation in the treated and untreated BPAN fibroblasts P1 and P2. The gene discussed is MAP1LC3B; the disease is neurodegeneration with brain iron accumulation 5.