To specifically evaluate the effect of dual JAG ligand KD exclusively on tumor burden, U266 cells transduced with a lentiviral vector expressing doxycycline-inducible shRNAs for JAG1 and JAG2 were injected in NGS mice, and JAG1 and JAG2 silencing was induced after MM cell engraftment. This evidence concerns the gene JAG2 and Miyoshi myopathy.