Among them, in the choline metabolism in cancer (ko05231) KEGG pathway, SLC22A1 and SLC44A1 were significantly upregulated in the cold season rumen epithelial, which transports choline and participate in the glycerophospholipid metabolism (ko00564) process, leading to a significant increase in phosphatidylcholine (Lecithin) levels during the cold season; LCAT, which regulates 1-Acyl-sn-glycero-3-phosphocholine levels, was significantly upregulated in the cold season. This evidence concerns the gene LCAT and cancer.