In the present study, 1,2-diacy-sn-glycerol-3P was significantly downregulated in the cold season and the regulatory downstream genes RAF1, WASF1 and WASF2 were significantly upregulated in the cold season, resulting in significant downregulation of mTOR and RPS6KB2, suggesting that chronic activation of the MAPK signaling pathway occurs in Tibetan sheep during the cold season, which can lead to the proliferation of tumor cells. Here, RAF1 is linked to neoplasm.