To further improve the specificity to HCC, Jain et al. compared the diagnostic power of methylation at different sites, revealing that P1 methylation had the best performance compared to E1 and P2 regions, and the sensitivity of P1 methylation of RASSF1A in AFP-negative HCC patients was up to 81.1% [56] (Fig. 4C). This evidence concerns the gene AFP and hepatocellular carcinoma.