At the same time, we found that in all the mutation sites of ORC1, R646L/W mutation frequency was the highest, including 1 case of LUAD, 1 case of LUAC, 1 case of RCC and 1 case of COAD (Fig. 3B), so the 3D map of R646LW site in ORC1 protein was also obtained (Fig. 3C). This evidence concerns the gene ORC1 and colon adenocarcinoma.