In our large cohort of 1606 adult AML patients, we found heterozygous SNVs to be the most common mode of alteration while we observed only four frame-shift mutations and only one small deletion of IKZF1. In accordance with previous results [37, 39], we also identified a mutational hotspot in the second N-terminal zinc finger domain at p.N159S, which was present in 19 cases (42.2%). This evidence concerns the gene IKZF1 and acute myeloid leukemia.