Analysis of RNA-seq data of Cdk4−/− and Cdk6−/− cancer cells revealed that the MCM and DNA polymerases are downregulated in Cdk4−/− cells while DNA polymerases were decreased in Cdk6−/− cells (Fig. 7d), which was validated by qPCR assay (Fig. 7e). The gene discussed is CDK6; the disease is cancer.