In order to find out which gene is more critical in Cdk4−/− medicated anti-tumor responses, Cdk4−/−/Sting−/− and Cdk4−/−/Mavs−/− MCA205 cell lines were established by CRISPR/Cas9 technology with specific sg-RNA pairs (Fig. 4a, b). Here, CDK4 is linked to neoplasm.