The results of in vitro experiments showed that Ecliptae Herba and its 3 active components significantly inhibited the expression of p-Smad2/3 protein induced by TGF-β1, as well as the proliferation, migration and invasion of MB231, suggesting that Ecliptae Herba has a regulatory effect on the growth and metastasis of breast cancer cells. The gene discussed is TGFB1; the disease is breast carcinoma.