To compare NUCB2 expression between glial and GBM cells, the mRNA levels of NUCB2 were measured in the glial cell line (SVGp12) and various GBM cell lines (GBM8401, U87-MG, G5T/VGH, M059K, Hs683, and A172) using real-time polymerase chain reaction. The gene discussed is NUCB2; the disease is glioblastoma.