To assess whether SH-SY5Y neuroblastoma cells have a similar distribution of ATM in subcellular fractions as adult cerebellar tissue, differential detergent isolation of nuclear, mitochondrial, and cytoplasmic fractions was performed firstly in cerebella from WT versus ATM-null mice at the age of 3.5 months (Figure 3a), and secondly in SH-SY5Y NT CTRL cells compared to shATM cells (Figure 3b). The gene discussed is ATM; the disease is neuroblastoma.