Nevertheless, after bafilomycin A1 cells treatment, which blocks autophagosome degradation and thus allows the measurement of autophagosome synthesis, a gradual and significant increase was found between basal condition and 8 h bafilomycin A1 treatment for p62 protein levels (p = 0.04 for controls; p = 0.02 for FXTAS patients) (Figure 2A). Here, SQSTM1 is linked to fragile X-associated tremor/ataxia syndrome.