Therefore, an insulin resistance mouse model was established in this study to assess the expression levels of several related biomarkers, i.e. INSR, insulin receptor substrate-1 (IRS-1) and phospho-IRS-1 (p-IRS-1), in the liver, skeletal muscle and ovary at different time points to understand the insulin signalling changes in different organs under insulin resistance. Here, INSR is linked to Insulin resistance.