However, the same perfusion flow rate (0.2 mL/min) and different values of shear stress (1.7 × 10−3–17 × 10−3 Pa) revealed shear stress dependency of both IGF1 production and cell sensitivity to IGF-1R inhibitors: higher shear stresses led to the increased production of IGF1 and lower sensitivity of Ewing sarcoma cells to IGF-1R inhibitor. The gene discussed is IGF1; the disease is Ewing sarcoma.