GCKR and obesity due to melanocortin 4 receptor deficiency: All samples were stratified and grouped into three categories including normal weight (BMI ≤25), overweight (BMI 25 to ≤30), and obese (BMI >30) to analyze the association of obesity with the GCKR (rs1260326) genetic variants in nonalcoholic fatty liver disease (NAFLD), type 2 diabetic mellitus (T2DM), and control subjects.