However, to our surprise, IGF2BP1 expression did not affect transcriptional outcome of Wnt/β-catenin signaling (assessed by TOP/FOP FLASH assay) in either non-transformed or CRC cells (Figure 1C; Figure 3C), suggesting that in our experimental settings IGF2BP1 does not regulate β-catenin/Tcf-dependent transcription. The gene discussed is IGF2BP1; the disease is colorectal carcinoma.