WNT3A and colorectal carcinoma: To investigate if our findings about the role of IGF2BP1 in regulating Wnt signaling in CRC are also applicable for non-transformed cells with the physiological Wnt/β-catenin signaling, we employed mouse embryonic fibroblasts (MEFs) with Igf2bp1−/− gene knockout (Igf2bp1-KO), and a recombinant Wnt-3a ligand to study the role of Igf2bp1 upon Wnt induction in vitro.