The subsequent western blot results also showed that elevated expression of GSDMD-N, NLRP3 and cleaved caspase-1 in lung tissues seen in PAH model rats was abrogated by As-IV administration (Fig. 2D, E and F), indicating that AS-IV alleviated pyroptosis in pulmonary artery tissues. The gene discussed is CASP1; the disease is pulmonary arterial hypertension.