MCCC2 and colorectal carcinoma: To explore whether MCCC2 expression effects cell proliferation, invasion, and migration, we firstly checked MCCC2 expression level across the Expression Atlas in EMBL-EBI website and estimated the MCCC2 mRNA expression level in eight CRC cell lines (Additional file 1: Fig. S1A, B) the HCT116 cell line with medium MCCC2 level was chosen to construct MCCC2-expressing cell lines, i.e., stably expressing short hairpin RNAs (shRNAs, knockdown: KD, Fig. 1A) and FLAG-MCCC2 overexpressing plasmid (overexpressing: oeMCCC2, Fig. 1B).