In confirmation of our earlier results, liver RIPK1 was more strongly ubiquitinated in the NASH Af6LKO mice than in the NASH Af6flox/flox animals (Fig. 6d), while signaling via the necroptotic pathway in liver tissue was decreased, as evidence by decreased p-MLKL levels (Fig. 6e). Here, MLKL is linked to metabolic dysfunction-associated steatohepatitis.