We show the utility of this platform inmeasuring N-terminal propeptide B-type natriuretic peptide (NT-proBNP),a heart failure biomarker, in complex medium and patient samples.We report a limit of detection of ∼0.05 ng/mL and a linearresponse between 0.2 and 2 ng/mL in spiked plasma samples. This evidence concerns the gene NPPB and heart failure.